4ODM
Structure of SlyD from Thermus thermophilus in complex with S2-W23A peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9199 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 184.120, 41.200, 131.520 |
| Unit cell angles | 90.00, 123.13, 90.00 |
Refinement procedure
| Resolution | 29.111 - 1.750 |
| R-factor | 0.1777 |
| Rwork | 0.176 |
| R-free | 0.20830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.094 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.111 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 83454 | |
| <I/σ(I)> | 18.31 | 2.42 |
| Completeness [%] | 98.9 | 97.6 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 19% PEG3350, 8% Tacsimate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
