4OBV
Ruminococcus gnavus tryptophan decarboxylase RUMGNA_01526 (alpha-FMT)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1159 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 135.030, 135.030, 249.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.442 - 2.840 |
| R-factor | 0.211 |
| Rwork | 0.210 |
| R-free | 0.24230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.543 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Refinement software | PHENIX ((phenix.refine: dev_1260)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.442 | 2.991 |
| High resolution limit [Å] | 2.840 | 2.840 |
| Number of reflections | 55229 | |
| Completeness [%] | 99.9 | 99.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.25 | 298 | 30% ethoxyethanol, 0.1M citrate pH 5.25, 4% polypropylene P400, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
