4OAU

Complete human RNase L in complex with biological activators.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	77
Detector technology	CCD
Collection date	2013-01-01
Detector	ADSC QUANTUM 315
Wavelength(s)	1.075
Spacegroup name	P 21 2 21
Unit cell lengths	60.130, 116.600, 162.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.442 - 2.600
R-factor	0.2133
Rwork	0.211
R-free	0.25120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.788
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	53.442
High resolution limit [Å]	2.600
Number of reflections	28947
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	277	RNase L (21-719) (15 mg/ml in buffer containing 20 mM HEPES pH 7.5, 109 mM NaCl, 5 mM MgCl 2, 5 mM DTT, 2.8 mM ATP or AMP-PCP, and 10% glycerol) was mixed with 2-5A and RNA18 at molar ratio 1:1.5:1.5, VAPOR DIFFUSION, temperature 277K