4O9U
Mechanism of transhydrogenase coupling proton translocation and hydride transfer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.12709 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 175.331, 160.590, 139.624 |
| Unit cell angles | 90.00, 128.36, 90.00 |
Refinement procedure
| Resolution | 38.900 - 6.926 |
| R-factor | 0.21598 |
| Rwork | 0.213 |
| R-free | 0.28372 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.102 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 38.900 |
| High resolution limit [Å] | 6.926 |
| Number of reflections | 4922 |
| Completeness [%] | 98.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 50 mM MgCl2, 0.5 M trimethylamine N-oxide, 26% polyethylenglycol 400, 2% octyl glucoside, 0.1mM NADP, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
