4O63

Co-enzyme Induced Conformational Changes in Bovine Eye Glyceraldehyde 3-Phosphate Dehydrogenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	79.752, 126.205, 83.228
Unit cell angles	90.00, 118.13, 90.00

Refinement procedure

Resolution	46.860 - 1.930
R-factor	0.19004
Rwork	0.188
R-free	0.23352
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j0x
RMSD bond length	0.018
RMSD bond angle	1.905
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.930
Rmerge	0.088
Number of reflections	108672
Completeness [%]	99.3
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	277	20% PEG3350 and 0.2M succinate, pH 7.0, VAPOR DIFFUSION, temperature 277K