4O63
Co-enzyme Induced Conformational Changes in Bovine Eye Glyceraldehyde 3-Phosphate Dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.752, 126.205, 83.228 |
| Unit cell angles | 90.00, 118.13, 90.00 |
Refinement procedure
| Resolution | 46.860 - 1.930 |
| R-factor | 0.19004 |
| Rwork | 0.188 |
| R-free | 0.23352 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j0x |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.905 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.930 |
| Rmerge | 0.088 |
| Number of reflections | 108672 |
| Completeness [%] | 99.3 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 277 | 20% PEG3350 and 0.2M succinate, pH 7.0, VAPOR DIFFUSION, temperature 277K |
