4O4R
Murine Norovirus RdRp in complex with PPNDS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97625 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.710, 197.430, 109.570 |
| Unit cell angles | 90.00, 114.06, 90.00 |
Refinement procedure
| Resolution | 59.360 - 2.400 |
| R-factor | 0.19439 |
| Rwork | 0.192 |
| R-free | 0.24870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uqs |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.389 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.400 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 90345 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | macrobatch | 8.4 | 293.15 | 1.6M (NH4)2SO4, 12% glycerol, 100mM TRIS/HCl, pH 8.4, macrobatch, temperature 293.15K |
