4NXP
Crystal Structure of Free T-cell Lymphoma Invasion and Metastasis-1 PDZ Domain Quadruple Mutant (QM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-07-21 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 45.843, 45.843, 71.362 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.850 - 2.300 |
| R-factor | 0.21873 |
| Rwork | 0.217 |
| R-free | 0.25777 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kzd |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.098 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.850 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.104 | 0.421 |
| Number of reflections | 3866 | |
| <I/σ(I)> | 7.7 | 2.1 |
| Completeness [%] | 93.9 | 97.3 |
| Redundancy | 3.18 | 3.17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 291 | 1.5M Ammonium Sulfate, 0.1M Tris, pH=8.5, 12% Glycerol, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
