4NV1
Crystal structure of a 4-N formyltransferase from Francisella tularensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-12 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.942, 80.065, 109.852 |
| Unit cell angles | 71.72, 88.62, 89.78 |
Refinement procedure
| Resolution | 29.960 - 2.100 |
| R-factor | 0.19536 |
| Rwork | 0.191 |
| R-free | 0.27038 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.293 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.070 | 0.231 |
| Number of reflections | 121975 | |
| <I/σ(I)> | 24 | 3.9 |
| Completeness [%] | 90.2 | 82.9 |
| Redundancy | 2.1 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 12-15% PEG8000, 100 mM HEPPS, 5 mM dTDP-Qui4NFo, 5 mM N5-THF, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
