4NV0
Crystal structure of cytosolic 5'-nucleotidase IIIB (cN-IIIB) bound to 7-methylguanosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-01-19 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.92 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.670, 99.110, 74.120 |
Unit cell angles | 90.00, 90.99, 90.00 |
Refinement procedure
Resolution | 41.194 - 1.650 |
R-factor | 0.1608 |
Rwork | 0.159 |
R-free | 0.19420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g08 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.580 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.670 | 1.750 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.053 | 0.547 |
Number of reflections | 76459 | |
<I/σ(I)> | 15.5 | 2.17 |
Completeness [%] | 94.5 | 81.7 |
Redundancy | 3.1 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 26% PEG 4000, 0.1M HEPES, 0.2M NaCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |