4NRV
Crystal Structure of non-edited human NEIL1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-11-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 |
| Unit cell lengths | 132.760, 132.760, 50.594 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.997 - 2.601 |
| R-factor | 0.1828 |
| Rwork | 0.178 |
| R-free | 0.23200 |
| Structure solution method | DIFFERENCE FOURIER METHODS |
| Starting model (for MR) | 1tdh |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.633 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 16.000 | 16.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 5.530 | 2.600 |
| Rmerge | 0.079 | 0.036 | 0.409 |
| Number of reflections | 9829 | ||
| <I/σ(I)> | 11.4 | ||
| Completeness [%] | 96.4 | 99.9 | 77.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 285 | 35% w/v PEG3350, 0.25 M lithium sulfate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 285K |
