4NRK

Structure of hemagglutinin with F95Y mutation of influenza virus B/Lee/40 complex with LSTc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	200
Detector technology	CCD
Collection date	2013-08-09
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872A
Spacegroup name	P 21 2 21
Unit cell lengths	83.610, 128.300, 211.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.873 - 2.630
R-factor	0.2085
Rwork	0.207
R-free	0.24480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.839
Refinement software	PHENIX ((phenix.refine: dev_1452))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.460	2.770
High resolution limit [Å]	2.630	2.630
Rmerge	0.111	0.499
Number of reflections	68564
<I/σ(I)>	11.5	2.4
Completeness [%]	100.0	100
Redundancy	5.3	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	290	0.1 M di-Sodium succinate 15% w/v PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K