4NR1
Factor inhibiting HIF-1 alpha in complex with consensus ankyrin repeat domain-(d)allyl-GLY peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-04-21 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.91730 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 86.370, 86.370, 148.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.690 - 2.680 |
| Rwork | 0.164 |
| R-free | 0.20960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h2k |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 74.690 | 2.750 | |
| High resolution limit [Å] | 2.680 | 2.680 | |
| Total number of observations | 12775 | 12453 | |
| Number of reflections | 16485 | ||
| <I/σ(I)> | 17.3 | 2.9 | |
| Completeness [%] | 99.9 | 99.9 | |
| Redundancy | 10 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1.6M AMMONIUM SULPHATE, 6% PEG 400, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
