4NQL
The crystal structure of the DUB domain of AMSH orthologue, Sst2 from S. pombe, in complex with lysine 63-linked diubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-11-09 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.489, 56.740, 135.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.470 - 2.300 |
| R-factor | 0.21587 |
| Rwork | 0.213 |
| R-free | 0.26333 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Model generated from PDB Entries 4MSQ (Sst2) and 2ZNV (diubiquitin) |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.163 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.430 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.380 | 2.300 |
| Rmerge | 0.112 | 0.458 | 0.549 |
| Number of reflections | 17629 | ||
| <I/σ(I)> | 17.8 | 5 | 4.1 |
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 6.8 | 6.8 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
