4NQL

The crystal structure of the DUB domain of AMSH orthologue, Sst2 from S. pombe, in complex with lysine 63-linked diubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-11-09
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.033
Spacegroup name	P 21 21 21
Unit cell lengths	49.489, 56.740, 135.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.470 - 2.300
R-factor	0.21587
Rwork	0.213
R-free	0.26333
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Model generated from PDB Entries 4MSQ (Sst2) and 2ZNV (diubiquitin)
RMSD bond length	0.006
RMSD bond angle	1.163
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	60.000	2.430	2.340
High resolution limit [Å]	2.300	2.380	2.300
Rmerge	0.112	0.458	0.549
Number of reflections	17629
<I/σ(I)>	17.8	5	4.1
Completeness [%]	100.0	100	100
Redundancy	6.8	6.8	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1