4NPQ
The resting-state conformation of the GLIC ligand-gated ion channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9725 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 131.533, 384.095, 148.439 |
| Unit cell angles | 90.00, 98.47, 90.00 |
Refinement procedure
| Resolution | 20.000 - 4.350 |
| R-factor | 0.2403 |
| Rwork | 0.240 |
| R-free | 0.24430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hfi |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.900 | 4.850 | 4.590 |
| High resolution limit [Å] | 4.350 | 4.600 | 4.350 |
| Rmerge | 0.084 | 0.835 | 1.580 |
| Number of reflections | 95802 | ||
| <I/σ(I)> | 10.8 | 2 | 1 |
| Completeness [%] | 99.6 | 99.8 | 99.8 |
| Redundancy | 4.1 | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 13-15% PEG4000, 200mM KSCN, 10mM CaCl2, 3% Trimethylamine-N-oxyde dihydrate, 0.1M Na Hepes pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
