4NPK
Extended-Synaptotagmin 2, C2A- and C2B-domains, calcium bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97912 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.840, 53.194, 85.276 |
| Unit cell angles | 90.00, 99.95, 90.00 |
Refinement procedure
| Resolution | 28.986 - 2.552 |
| R-factor | 0.2249 |
| Rwork | 0.222 |
| R-free | 0.28580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4npj |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.205 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.058 | 0.276 |
| Number of reflections | 12318 | |
| <I/σ(I)> | 33.4 | 2.45 |
| Completeness [%] | 88.7 | 38 |
| Redundancy | 3.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 6% PEG 8000, 0.1 M Hepes pH 8.0, 0.125 M NaCl, 2.5% glycerol, 0.02 M CaCl2, 0.5 mM TCEP, vapor diffusion, hanging drop, temperature 293K |
