4NOV
Xsa43E, a GH43 family enzyme from Butyrivibrio proteoclasticus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-08-05 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.100, 76.520, 82.520 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.080 - 1.330 |
R-factor | 0.17786 |
Rwork | 0.177 |
R-free | 0.18881 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.222 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 56.200 |
High resolution limit [Å] | 1.330 |
Number of reflections | 82628 |
Completeness [%] | 95.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.4 | 291 | 20% PEG8000, 0.2M NaCl, 0.1M Phosphate Citrate buffer , pH 3.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |