4NN7
Cytokine receptor complex - Crystal form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.968600 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.789, 50.105, 249.864 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.127 - 3.775 |
| R-factor | 0.277 |
| Rwork | 0.277 |
| R-free | 0.28650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nn5 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.872 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.3_1479)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.000 |
| High resolution limit [Å] | 3.770 | 3.770 |
| Rmerge | 0.267 | 0.778 |
| Number of reflections | 4968 | |
| <I/σ(I)> | 9.17 | 3 |
| Completeness [%] | 99.5 | 97.7 |
| Redundancy | 11.9 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 25% pentaerythritol ethoxylate, 0.1 M Tris-HCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
