4NN5

Cytokine receptor complex - Crystal form 1A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976293
Spacegroup name	P 21 21 2
Unit cell lengths	150.060, 79.756, 52.138
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.250 - 1.898
R-factor	0.1713
Rwork	0.170
R-free	0.20090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3di2
RMSD bond length	0.011
RMSD bond angle	1.218
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.3_1479))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.250	2.010
High resolution limit [Å]	1.898	1.898
Rmerge	0.086	0.670
Number of reflections	50292
<I/σ(I)>	19.9	3.9
Completeness [%]	99.7	98.4
Redundancy	13.2	13.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.1	293	14% PEG4000, 0.3 M sodium acetate, pH 4.5, 0.3 M calcium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 293K