4NM8
Crystal structure of broadly neutralizing antibody CR8043 bound to H3 influenza hemagglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2012-03-22 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 241.491, 142.355, 170.725 |
| Unit cell angles | 90.00, 133.46, 90.00 |
Refinement procedure
| Resolution | 43.821 - 4.004 |
| R-factor | 0.2423 |
| Rwork | 0.240 |
| R-free | 0.29240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fnk 4nm4 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.227 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.200 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.201 | 0.826 |
| Number of reflections | 34622 | |
| <I/σ(I)> | 6.4 | 2.2 |
| Completeness [%] | 97.7 | 90.8 |
| Redundancy | 6.8 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 2.2 M ammonium sulfate, 0.1 M sodium acetate pH 5.5, 3% PEG 400, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
