4NM8
Crystal structure of broadly neutralizing antibody CR8043 bound to H3 influenza hemagglutinin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2012-03-22 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97950 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 241.491, 142.355, 170.725 |
Unit cell angles | 90.00, 133.46, 90.00 |
Refinement procedure
Resolution | 43.821 - 4.004 |
R-factor | 0.2423 |
Rwork | 0.240 |
R-free | 0.29240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fnk 4nm4 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.227 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 4.200 |
High resolution limit [Å] | 4.000 | 4.000 |
Rmerge | 0.201 | 0.826 |
Number of reflections | 34622 | |
<I/σ(I)> | 6.4 | 2.2 |
Completeness [%] | 97.7 | 90.8 |
Redundancy | 6.8 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 2.2 M ammonium sulfate, 0.1 M sodium acetate pH 5.5, 3% PEG 400, VAPOR DIFFUSION, SITTING DROP, temperature 277K |