4NM7

Crystal structure of GSK-3/Axin complex bound to phosphorylated Wnt receptor LRP6 e-motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-31
Detector	PSI PILATUS 6M
Spacegroup name	P 61 2 2
Unit cell lengths	82.002, 82.002, 280.345
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.350 - 2.300
R-factor	0.185
Rwork	0.183
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1o9u
RMSD bond length	0.004
RMSD bond angle	0.759
Data reduction software	XDS
Data scaling software	Aimless (0.1.26)
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.352	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	25932
<I/σ(I)>	17.8	0.9
Completeness [%]	100.0	100
Redundancy	18.7	18.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	277	10% PEG 35,000, 20mM Tris 7.5, 300mM NaCl, 5% glycerol, 10mM MgCl2, 200uM ATP, and 5mM DTT, MICRODIALYSIS, temperature 277K