4NL8

PriA Helicase Bound to SSB C-terminal Tail Peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-25
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	106.610, 153.248, 192.373
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 4.080
R-factor	0.24341
Rwork	0.240
R-free	0.29848
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.317
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (MR)
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.170
High resolution limit [Å]	4.080	4.080
Number of reflections	21752
<I/σ(I)>	10.91	1.89
Completeness [%]	100.0	100
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.1 M ammonium acetate, 0.015 M magnesium acetate tetrahydrate, 0.05 M sodium cacodylate trihydrate pH 6.5, and 10% v/v 2-propanol, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K