4NGF
Structure of human Dicer Platform-PAZ-Connector Helix cassette in complex with 17-mer siRNA having 5'-p and UU-3' ends (3.1 Angstrom resolution)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.07500 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 99.245, 104.191, 368.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.478 - 3.101 |
R-factor | 0.2255 |
Rwork | 0.223 |
R-free | 0.27030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ngd |
RMSD bond length | 0.006 |
RMSD bond angle | 1.165 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.210 |
High resolution limit [Å] | 3.100 | 6.670 | 3.100 |
Rmerge | 0.120 | 0.056 | 0.650 |
Number of reflections | 32098 | ||
<I/σ(I)> | 7.4 | ||
Completeness [%] | 91.6 | 99.7 | 87.8 |
Redundancy | 13.1 | 13.5 | 13.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.08 M HEPES sodium, pH 7.5, 8% 2-propanol, 16% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |