4NBS
The structure of aIF2gamma subunit H20F from archaeon Sulfolobus solfataricus complexed with GDPCP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-04 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.91841 |
Spacegroup name | I 2 3 |
Unit cell lengths | 187.152, 187.152, 187.152 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.788 - 2.305 |
R-factor | 0.1787 |
Rwork | 0.177 |
R-free | 0.20140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3SJY |
RMSD bond length | 0.006 |
RMSD bond angle | 1.009 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_1389)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.790 | 2.360 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.576 | |
Number of reflections | 48037 | |
<I/σ(I)> | 2.41 | |
Completeness [%] | 100.0 | 99.8 |
Redundancy | 6.85 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 301 | 4M sodium formiate, 0.1M sodium cacodylate, 1% MMEPEG 5K, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 301K |