4N9L
crystal structure of beta-lactamse PenP_E166S in complex with meropenem
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-07-05 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.260, 90.960, 66.200 |
| Unit cell angles | 90.00, 104.24, 90.00 |
Refinement procedure
| Resolution | 37.100 - 2.300 |
| R-factor | 0.18354 |
| Rwork | 0.180 |
| R-free | 0.24187 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.996 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.480 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 22119 |
| Completeness [%] | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | Tris, PEG 3350, ammonium acetate, pH 8.0, vapor diffusion, hanging drop, temperature 289K |
