4N99

E. coli sliding clamp in complex with 6-chloro-2,3,4,9-tetrahydro-1H-carbazole-7-carboxylic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-08-10
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	41.010, 65.130, 73.240
Unit cell angles	72.95, 85.06, 85.24

Refinement procedure

Resolution	40.950 - 2.300
R-factor	0.2647
Rwork	0.261
R-free	0.33780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k3s
RMSD bond length	0.004
RMSD bond angle	0.877
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	69.874	40.949	2.420
High resolution limit [Å]	2.300	7.270	2.300
Rmerge		0.052	0.643
Total number of observations		3308	15628
Number of reflections	29744
<I/σ(I)>	8.5	11.6	1.2
Completeness [%]	92.9	95.8	89.9
Redundancy	3.8	3.4	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1