4N7V

Crystal structure of human Plk4 cryptic polo box (CPB) in complex with a Cep152 N-terminal fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-02
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	126.551, 63.942, 79.499
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.263 - 2.758
R-factor	0.2583
Rwork	0.258
R-free	0.28760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4g7n
RMSD bond length	0.004
RMSD bond angle	0.915
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.750	2.750
Rmerge	0.068	0.303
Number of reflections	15869
<I/σ(I)>	16.9	2.06
Completeness [%]	92.6	72.7
Redundancy	3.8	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295.15	22% PEG 4000, 3% Iso-propanol, 0.1M Tris-HCl, 0.2M Lithium Sulfate , pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K