4N7Q
Crystal structure of eukaryotic THIC from A. thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-01-26 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.97795 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 107.000, 107.000, 88.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.027 - 1.600 |
| R-factor | 0.1538 |
| Rwork | 0.152 |
| R-free | 0.17600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3epm |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.068 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CCP4 |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.050 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.146 | 1.271 |
| Number of reflections | 77101 | |
| <I/σ(I)> | 13.58 | 2.35 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10 | 9.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291.15 | 0.01 M cobalt (II) chloride hexahydrate, 0.1 M sodium acetate trihydrate, 1 M 1,6-Hexanediol, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K |
