4N5Y
Crystal structure of H5 hemagglutinin mutant (N158D, N224K and Q226L) from the influenza virus A/Viet Nam/1203/2004 (H5N1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-27 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.05876 |
Spacegroup name | P 1 |
Unit cell lengths | 117.915, 118.105, 273.799 |
Unit cell angles | 91.50, 90.18, 119.87 |
Refinement procedure
Resolution | 45.283 - 3.162 |
R-factor | 0.215 |
Rwork | 0.213 |
R-free | 0.26150 |
Structure solution method | Phaser |
Starting model (for MR) | 3gbm |
RMSD bond length | 0.012 |
RMSD bond angle | 1.555 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.270 |
High resolution limit [Å] | 3.162 | 3.162 |
Number of reflections | 199766 | |
<I/σ(I)> | 7.1 | |
Completeness [%] | 93.6 | 83.4 |
Redundancy | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.1 M HEPES, pH 7.5, 0.2 KF, 22% (w/v) PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K |