4N43

Human enterovirus 71 uncoating intermediate captured at atomic resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-03
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97914
Spacegroup name	P 42 3 2
Unit cell lengths	352.416, 352.416, 352.416
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.840 - 3.801
R-factor	0.2466
Rwork	0.245
R-free	0.27410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pov
RMSD bond length	0.002
RMSD bond angle	0.641
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.840	50.000	3.870
High resolution limit [Å]	3.801	10.290	3.801
Rmerge	0.384	0.170	0.839
Number of reflections	50069
<I/σ(I)>	2.3	19.48	1.34
Completeness [%]	68.0	56.1	73.4
Redundancy	5	5.6	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7	289	0.1M Cacodylate containing 1.6M Sodium Acetate, pH 7.0, vapor diffusion, hanging drop, temperature 289K