4N3S

Crystal structure of eukaryotic translation initiation factor eIF5B (399-852) from Saccharomyces cerevisiae, apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.826580
Spacegroup name	P 41
Unit cell lengths	118.010, 118.010, 77.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.949 - 1.832
R-factor	0.1689
Rwork	0.168
R-free	0.19410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g7r
RMSD bond length	0.008
RMSD bond angle	1.141
Data scaling software	XSCALE
Phasing software	PHASER (2.3.0)
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]			1.930
High resolution limit [Å]	1.830	50.000	1.830
Rmerge	0.039		0.525
Number of reflections	93360		13677
<I/σ(I)>	21.05		3.11
Completeness [%]	99.8		99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	5 % PEG 3350 and 20 mM MgCl2., VAPOR DIFFUSION, SITTING DROP, temperature 277K