4N0E
Crystal structure of the K345L variant of the Gi alpha1 subunit bound to GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-05 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.078 |
Spacegroup name | I 4 |
Unit cell lengths | 121.527, 121.527, 68.217 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.218 - 2.100 |
R-factor | 0.1862 |
Rwork | 0.184 |
R-free | 0.22030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4n0d |
RMSD bond length | 0.007 |
RMSD bond angle | 1.032 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.5.2) |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 85.933 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 29073 | |
Completeness [%] | 99.0 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 2.0 ammonium sulfite, pH 8.0, 5 mM MgSO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |