4N0D

Crystal structure of the K345L variant of the Gi alpha1 subunit bound to GTPgammaS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-23
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 32 2 1
Unit cell lengths	79.635, 79.635, 104.825
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.807 - 1.550
R-factor	0.1585
Rwork	0.157
R-free	0.18740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 2ZJY was used as the starting model.
RMSD bond length	0.018
RMSD bond angle	1.786
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.3_1479))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.966	1.610
High resolution limit [Å]	1.550	1.550
Number of reflections	55216
Completeness [%]	99.0	92

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	2.2 M ammonium sulfite, pH 8.0, 20 mM magnesium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K