4N07
Crystal structure of the GluA2 ligand-binding domain (S1S2J-L483Y-N754S) in complex with glutamate and BPAM-344 at 1.87 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 113.904, 164.034, 47.284 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.476 - 1.870 |
R-factor | 0.177 |
Rwork | 0.175 |
R-free | 0.21140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lb8 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.081 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.16) |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.476 | 28.476 | 1.970 |
High resolution limit [Å] | 1.870 | 5.910 | 1.870 |
Rmerge | 0.057 | 0.030 | 0.385 |
Total number of observations | 9383 | 43796 | |
Number of reflections | 74220 | ||
<I/σ(I)> | 15.2 | 20.6 | 2 |
Completeness [%] | 99.9 | 97.2 | 100 |
Redundancy | 4.1 | 3.8 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 16.5% PEG4000, 0.15 M zinc acetate, 0.1 M cacodylate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |