4MZG

Crystal structure of human Spindlin1 bound to histone H3K4me3 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	77
Detector technology	CCD
Collection date	2012-06-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9794
Spacegroup name	P 1 21 1
Unit cell lengths	43.168, 122.037, 49.837
Unit cell angles	90.00, 91.71, 90.00

Refinement procedure

Resolution	32.144 - 1.698
R-factor	0.184
Rwork	0.183
R-free	0.20710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.168
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	2.220	1.730
High resolution limit [Å]	1.698	2.140	1.698
Rmerge	0.057	0.088	0.645
Number of reflections	56230
<I/σ(I)>	24.8	19.3	2.64
Completeness [%]	99.6	100	99.6
Redundancy	4.4	4.4	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291.15	37.5% MPD_P1K_P3350, 0.1M bicine/Trizma, pH 8.5, 0.06M MgCl2/CaCl2, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K