4MXV

Structure of Lymphotoxin alpha bound to anti-LTa Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-08-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	209.847, 209.847, 130.142
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.796 - 3.200
R-factor	0.1807
Rwork	0.177
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1TNR AND 1FVE
RMSD bond length	0.010
RMSD bond angle	1.303
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.310
High resolution limit [Å]	3.200	6.890	3.200
Number of reflections	46756
Completeness [%]	100.0	99.9	100
Redundancy	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	12% PEG6000, 2 M sodium chloride, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K