4MVT
Crystal structure of SUMO E3 Ligase PIAS3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.450, 85.440, 89.530 |
| Unit cell angles | 83.08, 86.57, 86.14 |
Refinement procedure
| Resolution | 48.040 - 2.300 |
| R-factor | 0.239 |
| Rwork | 0.238 |
| R-free | 0.26980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fo9 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP (11.0) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 6.240 | 2.300 |
| Rmerge | 0.137 | 0.080 | 0.950 |
| Number of reflections | 71156 | ||
| <I/σ(I)> | 18.1 | ||
| Completeness [%] | 98.4 | 98.6 | 97.6 |
| Redundancy | 5.1 | 4.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 291 | 20% PEG 8000, 0.2 M Magnesium Chloride, 0.1 M Tris pH8.5,(1:800-1:1300) w/w chymotrypsin, vapor diffusion hanging drop, temperature 291K |
