4MUM

Crystal structure of mitochondrial 5'(3')-deoxy ribonucleotidase alternative spliced variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-01-31
Detector	RAYONIX MX-225
Wavelength(s)	0.97826
Spacegroup name	P 43 21 2
Unit cell lengths	73.372, 73.372, 106.672
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.656 - 1.271
R-factor	0.1321
Rwork	0.131
R-free	0.14920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4l6a
RMSD bond length	0.020
RMSD bond angle	2.336
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.656	46.656	1.350
High resolution limit [Å]	1.271	3.800	1.271
Rmerge	0.036	0.020	0.608
Number of reflections	76192	3151	11389
<I/σ(I)>	26.48	83.54	2.05
Completeness [%]	98.8	99.6	92.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	291	20 mM potassium phosphate monobasic, 8% PEG8000, 10% gycerol, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 291K