4MTH
Crystal structure of mature human RegIIIalpha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 30.762, 49.528, 92.152 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.130 - 1.470 |
R-factor | 0.187 |
Rwork | 0.185 |
R-free | 0.21000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.029 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.470 |
Number of reflections | 24610 |
<I/σ(I)> | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 22% PEG 8000, 0.1M MES pH 6.0, 0.1M NACL, 10MM NAAC, 5% GLYCEROL, VAPOR DIFFUSION, HANGING DROP, temperature 293K |