4MRM
Crystal structure of the extracellular domain of human GABA(B) receptor bound to the antagonist phaclofen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.070, 111.940, 73.470 |
| Unit cell angles | 90.00, 97.67, 90.00 |
Refinement procedure
| Resolution | 47.210 - 2.860 |
| R-factor | 0.2095 |
| Rwork | 0.208 |
| R-free | 0.23190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mqe |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.910 |
| High resolution limit [Å] | 2.860 | 7.750 | 2.860 |
| Rmerge | 0.072 | 0.022 | 0.629 |
| Number of reflections | 25855 | ||
| <I/σ(I)> | 10.1 | ||
| Completeness [%] | 99.9 | 100 | 99.1 |
| Redundancy | 3.8 | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 10% PEG3350, 20% glycerol, 0.12 M sodium acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
