4MQU
Human GKRP complexed to AMG-3969 and S6P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | RIGAKU SATURN 92 |
| Spacegroup name | P 65 |
| Unit cell lengths | 149.066, 149.066, 132.463 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.800 - 2.220 |
| R-factor | 0.21575 |
| Rwork | 0.212 |
| R-free | 0.27676 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Internal structures |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.758 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.800 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 58853 |
| Completeness [%] | 93.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Well buffer: 0.1M Bis-tris, 0.2M NaI, 16% PEG 8K, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
