4MNG
Structure of the DP10.7 TCR with CD1d-sulfatide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-03-24 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.03321 |
| Spacegroup name | P 32 |
| Unit cell lengths | 229.333, 229.333, 52.646 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.652 - 3.006 |
| R-factor | 0.1794 |
| Rwork | 0.178 |
| R-free | 0.20680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4MNH and 4MQ7 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.795 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 61100 | |
| Completeness [%] | 99.2 | 95.7 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 11% PEG 4000 5% isopropanol 2 mM glutathione reduced/oxidized 50 mM tri-sodium citrate, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
