4ML0
Crystal structure of E.coli DinJ-YafQ complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 175.901, 120.250, 120.201 |
| Unit cell angles | 90.00, 130.40, 90.00 |
Refinement procedure
| Resolution | 43.713 - 2.100 |
| R-factor | 0.1748 |
| Rwork | 0.175 |
| R-free | 0.21510 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.063 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.102 | 0.458 |
| Number of reflections | 110921 | |
| <I/σ(I)> | 33 | 9.32 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 4.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2M (NH4)2SO4, 2.5% 2-Propanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
