4MHX
Crystal Structure of Sulfamidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99989 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.380, 107.900, 79.790 |
| Unit cell angles | 90.00, 104.07, 90.00 |
Refinement procedure
| Resolution | 44.300 - 2.000 |
| R-factor | 0.1939 |
| Rwork | 0.192 |
| R-free | 0.22990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.564 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.3.0) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 2.040 | ||
| High resolution limit [Å] | 1.990 | 8.910 | 1.990 |
| Rmerge | 0.089 | 0.011 | 0.011 |
| Number of reflections | 134186 | 1477 | 8986 |
| <I/σ(I)> | 7.83 | 22.62 | 1.06 |
| Completeness [%] | 98.7 | 97.1 | 88.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 25% (w/v) polyethylene glycol (PEG) 3350, 200 mM MgCl2 and 100 mM HEPES buffer, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
