4MHE
Crystal structure of CC-chemokine 18
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.075, 55.168, 60.377 |
| Unit cell angles | 90.00, 109.25, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.100 |
| R-factor | 0.1803 |
| Rwork | 0.176 |
| R-free | 0.21810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x69 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.061 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.033 |
| High resolution limit [Å] | 2.100 |
| Number of reflections | 19065 |
| Completeness [%] | 99.9 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291.15 | 0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dihydrate, pH 5.6, 30% w/v Polyethylene glycol 4,000, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |
