4MH8
The crystal structure of the monomeric reverse transcriptase from moloney murine leukemia virus
Replaces: 1RW3Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 108 |
Detector technology | CCD |
Collection date | 2001-06-28 |
Detector | CUSTOM-MADE |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 242.332, 94.621, 52.408 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.636 - 3.000 |
R-factor | 0.2372 |
Rwork | 0.235 |
R-free | 0.27690 |
Structure solution method | MIRAS AND MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qaj 4hkq |
RMSD bond length | 0.004 |
RMSD bond angle | 0.783 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 22526 | |
Completeness [%] | 89.8 | |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | POTASSIUM CHLORIDE, MAGNESIUM ACETATE, SODIUM CACODYLATE, PEG 8000, PH 6.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K REMARK 300 |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | POTASSIUM CHLORIDE, MAGNESIUM ACETATE, SODIUM CACODYLATE, PEG 8000, PH 6.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K REMARK 300 |