4MH8

The crystal structure of the monomeric reverse transcriptase from moloney murine leukemia virus

Replaces:  1RW3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	108
Detector technology	CCD
Collection date	2001-06-28
Detector	CUSTOM-MADE
Spacegroup name	P 21 21 2
Unit cell lengths	242.332, 94.621, 52.408
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.636 - 3.000
R-factor	0.2372
Rwork	0.235
R-free	0.27690
Structure solution method	MIRAS AND MOLECULAR REPLACEMENT
Starting model (for MR)	1qaj 4hkq
RMSD bond length	0.004
RMSD bond angle	0.783
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.110
High resolution limit [Å]	3.000	3.000
Number of reflections	22526
Completeness [%]		89.8
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	POTASSIUM CHLORIDE, MAGNESIUM ACETATE, SODIUM CACODYLATE, PEG 8000, PH 6.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K REMARK 300
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	POTASSIUM CHLORIDE, MAGNESIUM ACETATE, SODIUM CACODYLATE, PEG 8000, PH 6.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K REMARK 300