4MEC
Crystal structure of RAT Heme oxygenase-1 in complex with ZN(II)-Protoporphyrin IX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-21 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.2000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.059, 73.167, 148.757 |
| Unit cell angles | 86.41, 87.62, 86.25 |
Refinement procedure
| Resolution | 38.940 - 3.200 |
| R-factor | 0.252 |
| Rwork | 0.249 |
| R-free | 0.29900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dve |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.865 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 27085 | |
| <I/σ(I)> | 14.4 | 2.7 |
| Completeness [%] | 97.8 | 94 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 30% PEG 4000, 0.2M Sodium Acetate, 0.1M Tris-HCL, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
