4MDK

Cdc34-ubiquitin-CC0651 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97919
Spacegroup name	P 1
Unit cell lengths	55.403, 72.220, 75.912
Unit cell angles	71.89, 79.63, 81.61

Refinement procedure

Resolution	44.627 - 2.610
R-factor	0.2069
Rwork	0.204
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2OB4 AND 3A33
RMSD bond length	0.008
RMSD bond angle	1.264
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge	0.079	0.044	0.457
Number of reflections	31394
<I/σ(I)>	13.7	34.3	1.2
Completeness [%]	95.8	97.2	85.5
Redundancy	2.2	2.2	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	28% PEG3350, 0.1 M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K