4MDA
Structure of Mos1 transposase catalytic domain and Raltegravir with Mn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Detector technology | PIXEL |
| Collection date | 2011-02-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 44.580, 44.580, 209.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.010 - 1.700 |
| R-factor | 0.1988 |
| Rwork | 0.197 |
| R-free | 0.24150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f7t |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.052 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.600 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.071 | 0.248 |
| Number of reflections | 24487 | |
| <I/σ(I)> | 16.7 | 6.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.8 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 290 | 22% w/v PEG4000, 100 mM Tris, pH 6.8, 5 mM manganese chloride, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
