4MD9
Crystal Structure of symmetric CK2 holoenzyme with mutated alpha subunit (F121E truncated at aa 336)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.873 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 283.924, 115.707, 208.223 |
| Unit cell angles | 90.00, 119.22, 90.00 |
Refinement procedure
| Resolution | 181.725 - 3.500 |
| R-factor | 0.23038 |
| Rwork | 0.229 |
| R-free | 0.25897 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 4DGL AND 4KWP |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.159 |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 181.725 | 48.306 | 3.690 |
| High resolution limit [Å] | 3.500 | 11.070 | 3.500 |
| Rmerge | 0.039 | 0.621 | |
| Number of reflections | 74256 | ||
| <I/σ(I)> | 6.6 | 12.5 | 1.2 |
| Completeness [%] | 99.7 | 97.2 | 99.9 |
| Redundancy | 3.3 | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 277 | 20% PEG3350, 0.2 M ammonium citrate, pH 6.5, VAPOR DIFFUSION, temperature 277K |
