4MBG

Crystal structure of Aspergillus fumigatus protein farnesyltransferase binary complex with farnesyldiphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-08
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 1 21 1
Unit cell lengths	63.252, 90.793, 83.138
Unit cell angles	90.00, 110.87, 90.00

Refinement procedure

Resolution	23.960 - 1.740
R-factor	0.124
Rwork	0.122
R-free	0.16400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	HOMOLOGY MODEL OF ASPERGILLUS FUMIGATUS PROTEIN FARNESYLTRANSFERASE GENERATED USING PHYRE
RMSD bond length	0.011
RMSD bond angle	1.231
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.740
Number of reflections	89198
Completeness [%]	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	290	4-10 % PEG6000, 600-800 mM LiCl, and 100 mM HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K